Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Detection of biogenic magnetic nanoparticles in rapidly dividing tumor cells by the nonlinear magnetization method

doi: 10.3389/fbioe.2025.1680057

Figure Lengend Snippet: Comparative assessment of ferric iron accumulation in C6-RFP-Qt and C6-RFP cells by Prussian blue staining. Experimental design (a) ; Perls stained C6-RFP-Qt (b) and C6-RFP (c) cells. Black arrows indicate ferric ferrocyanide in C6-RFP-Qt cells. Light microscopy, CX41 FLLED microscope, scale bars are 50 μm.

Article Snippet: The staining of the cells was carried out in accordance with the manufacturer’s guidelines of the Prussian blue iron Staining Kit (Biovitrum, Moscow, Russia).

Techniques: Staining, Light Microscopy, Microscopy

Journal: Materials Today Bio

Article Title: Analysis of the therapeutic effect and potential mechanism of Fe 3 O 4 -polydopamine nanoparticles in enhancing mesenchymal stem cells therapy for pulmonary fibrosis

doi: 10.1016/j.mtbio.2025.102098

Figure Lengend Snippet: Characterization and uptake of Fe 3 O 4 @PDA NPs by MSC. A. Schematic diagram illustrating the synthesis process of Fe 3 O 4 @PDA NPs. B. Transmission electron microscopy (TEM) image depicting the morphology of Fe 3 O 4 @PDA NPs. C. Scanning electron microscopy (SEM) image depicting the morphology of Fe 3 O 4 @PDA NPs. D. DLS size distribution analysis of Fe 3 O 4 @PDA NPs. E. TEM image illustrating the internalization of Fe 3 O 4 @PDA NPs by MSC. F. Quantification of iron concentration in MSC using inductively coupled plasma-optical emission spectroscopy (ICP-OES). G. Prussian blue staining of MSC treated with varying concentrations of Fe 3 O 4 @PDA NPs.

Article Snippet: Sections were stained using a Prussian blue iron stain kit (Servicebio, Wuhan, China), according to the manufacturer's instructions.

Techniques: Transmission Assay, Electron Microscopy, Concentration Assay, Clinical Proteomics, Spectroscopy, Staining

Journal: Materials Today Bio

Article Title: Analysis of the therapeutic effect and potential mechanism of Fe 3 O 4 -polydopamine nanoparticles in enhancing mesenchymal stem cells therapy for pulmonary fibrosis

doi: 10.1016/j.mtbio.2025.102098

Figure Lengend Snippet: Therapeutic effects of MSC and MSC + NPs in the BLM-induced PF mouse model. A. Schematic representation of the BLM-induced PF mouse model used in this study. B. Representative photograph showing lung morphology at the study endpoint. C. Prussian blue staining illustrating iron deposition in lung tissues. D. Histological assessment of lung tissues, including hematoxylin and eosin (H&E), Masson's trichrome, Picrosirius red, and periodic acid–Schiff (PAS) staining. E. Histogram showing the percentage of the positive area of Masson, Picrosirius red, and PAS staining. F. Hydroxyproline content in lung tissues. Data are presented as the mean ± standard deviation. Data are presented as the mean ± standard deviation. ns, no significant difference; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.

Article Snippet: Sections were stained using a Prussian blue iron stain kit (Servicebio, Wuhan, China), according to the manufacturer's instructions.

Techniques: Staining, Standard Deviation